A photo‐reactive derivative of ganglioside GM1 specifically cross‐links VIP21‐caveolin on the cell surface

FEBS Letters

Simons, Kai²  Fra, Anna M.²  Palestini, Paola¹  Masserini, Massimo²  Sonnino, Sandro¹ 

[1] Department of Medical Chemistry and Biochemistry, University of Milano, via Saldini 50, 20133 Milano, Italy;European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany

关键词: Caveolae;    Cross-linking;    Ganglioside GM1;    Sphingolipid;    VIP21-caveolin;    BSA;    bovine serum albumin;    CTB;    cholera toxin B sub-unit;    GPI;    glycosylphosphatidylinositol;    MDCK;    Madin-Darby canine kidney cell line;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(95)95228-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous studies have shown that sphingolipids may be enriched in caveolae, plasmalemmal invaginations implicated in endocytosis and signal transduction. We synthesised a radiolabeled derivative of ganglioside GM1 bearing a photo-reactive cross-linker at the end of its acyl chain. After insertion in the plasma membrane of cultured A431 or MDCK cells and photoactivation, the main protein cross-linked by the GM1 derivative was VIP21-caveolin, an essential structural component of caveolae. This result shows close proximity between GM1 molecules and VIP21-caveolin in the caveolar membrane and strongly implicates sphingolipid segregation in the biogenesis of caveolae.

【 授权许可】

Unknown