Ribozyme mediated cleavage of acute phase serum amyloid A (A‐SAA) mRNA in vitro

FEBS Letters

Gaughan, Derval J.¹  Steel, Diana M.¹  Whitehead, Alexander S.¹ 

[1] Department of Genetics and Biotechnology Institute, Trinity College, University of Dublin, Dublin, Ireland

关键词: Acute phase response;    Hammerhead ribozyme;    Inflammation;    Serum amyloid A;    A-SAA;    acute-phase serum amyloid A;    HDL;    high density lipoprotein;    SAA;    serum amyloid A;   

DOI  :  10.1016/0014-5793(95)01118-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The 1000-fold induction of acute phase serum amyloid A (A-SAA) in the liver during inflammation indicates that this protein plays an important, though ill-defined, role in host defence. Paradoxically, prolonged overproduction of A-SAA is a causative factor in secondary amyloidosis and possibly other diseases such as atherosclerosis; the ability to down-regulate A-SAA synthesis is therefore of considerable clinical importance. We have successfully generated anti-SAA hammerhead ribozymes and we report that they are capable of cleaving A-SAA mRNA in vitro.

【 授权许可】

Unknown