Specific RNA cleavages induced by manganese ions

FEBS Letters

Michałowski, Daniel¹  Ciesiołka, Jerzy¹  Krzyżosiak, Wlodzimierz J.¹  Wrzesinski, Jan¹ 

[1] Institute of Bioorganic Chemistry, Polish Academy of Sciences, ul. Noskowskiego 12, 61-704 Poznan, Poland

关键词: Manganese;    Ion-induced hydrolysis;    tRNA;    Transcription;    in vitro;    tRNA AC-arm mutants;   

DOI  :  10.1016/0014-5793(95)01077-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The specificity and efficiency of manganese ion-induced RNA hydrolysis was studied with several tRNA molecules. In case of yeast tRNA^Phe, the main cleavage occurs at p16 and minor cuts at p17–18, p20–21, p34 and p36–37. The major Mn(II)-induced cut in yeast elongator tRNA^Met is also located in the D-loop at p16 and it is stronger than that observed in tRNA^Phe. In initiator tRNA^Met from yeast two strong Mn(II) cleavages of equal intensity occur at p16 and p17. This is in contrast with single, much weaker cuts induced in the D-loop of that tRNA by Mg(II), Eu(III) and Pb(II) ions. Interestingly, in case of yeast tRNA^Glu the main cleavage caused by Mn(II), Mg(II) and Pb(II) ions occurs in the anticodon loop. The involvement of hypermodified base mnm⁵s²U in this cleavage was ruled out based on results obtained with in vitro transcript of yeast tRNA^Glu anticodon arm. Mutation of a single base A37G in the anticodon loop of the transcript drastically reduced the specificity of Mn(II)-induced hydrolysis.

【 授权许可】

Unknown