Apoptosis, DNA damage and ubiquitin expression in normal and mdx muscle fibers after exercise

FEBS Letters

Franceschi, Claudio¹  Arslan, Paola²  Podhorska-Okolov, Marzena³  Rizzi, Corrado³  Sandri, Marco³  Carraro, Ugo³  Monti, Daniela¹ 

[1]Department of Biomedical Sciences, General Pathology Section, University of Modena, via Campi 287, 41100 Modena, Italy

[2]Institute of Experimental and Laboratory Medicine, University of Padova, 35100 Padova, Italy

[3]C.N.R. Unit for Muscle Biology and Physiopathology, Department of Biomedical Sciences, University of Padova, via Trieste 75, 35231 Padova, Italy

关键词: Apoptosis;    Ubiquitin;    Muscle damage;    EDL;    Extensor Digitorum Longus;    PBS;    phosphate-buffered saline;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide gel electrophoresis;    ROS;    reactive oxygen species;   

DOI  :  10.1016/0014-5793(95)00908-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The current view indicates that after eccentric exercise myofibers are mechanically damaged and therefore an inflammatory and necrotic process occurs. In the present paper we examine the possibility that apoptosis plays a role in normal and dystrophin-deficient muscles after running. We analysed for apoptosis normal and dystrophin-deficient mouse muscles after a night of spontaneous wheel-running followed by two days of rest. Terminal deoxynucleotidyl transferase-mediated endlabeling of DNA in nuclei in tissue sections and gel electrophoresis of extracted DNA showed the presence of fragmented DNA. Furthermore, ubiquitin, a protein whose appearance is related to apoptosis, increased in muscles of both dystrophic and normal runner mice. The present findings which confirm that DNA damage is absent in muscles of sedentary mice but present in muscles of runner mice offer a new hypothesis on early events of muscle damage.

【 授权许可】

Unknown