Cloning and sequencing of a human thioredoxin reductase

FEBS Letters

Gasdaska, John R.¹  Gasdaska, Pamela Y.¹  Cochran, Shawn¹  Powis, Garth¹ 

[1] Arizona Cancer Center, 1515 N. Campbell Avenue, Tucson, AZ 85724, USA

关键词: Thioredoxin reductase;    Human placenta;   

DOI  :  10.1016/0014-5793(95)01003-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The DNA sequence encoding human placental thioredoxin reductase has been determined. Of the 3826 base pairs sequenced, 1650 base pairs were in an open reading frame encoding a mature protein with 495 amino acids and a calculated molecular mass of 54,171. Sequence analysis showed strong similarity to glutathione reductases and other NADPH-dependent reductases. Human thioredoxin reductase contains the redoxactive cysteines in the putative FAD binding domain and has a dimer interface domain not previously seen with prokaryote and lower eukaryote thioredoxin reductases.

【 授权许可】

Unknown