Isolation and characterization of syncytiotrophoblast plasma membrane from human placenta

FEBS Letters

Khalfoun, B.³  Bardos, P.³  Gutman, N.¹  Arbeille-Brassart, B.²  Degenne, D.³ 

[1] Laboratoire de Biochimie, Faculté de Médecine, 2 bis Bd Tonnelé, 37032 Tours Cédex, France;Laboratoire de Microscopie Electronique, Faculté de Médecine, 2 bis Bd Tonnelé, 37032 Tours Cédex, France;Laboratoire d'Immunologie, Faculté de Médecine, 2 bis Bd Tonnelé, 37032 Tours Cédex, France

关键词: Plasma membrane isolation;    Syncytiotrophoblast;    Human placenta;   

DOI  :  10.1016/0014-5793(85)81108-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human full-term syncytiotrophoblast plasma membranes isolated by mechanical procedures (sieving and ultrasonic disintegration), purified by phase centrifugation, form a single band of 1.052 ± 0.002 density in percoll gradient. The purity of the preparation was assessed by electron microscopy, enzyme analysis and β₂-microglobulin determination.

【 授权许可】

Unknown