Isolation of a novel ras‐recision gene that is induced by hydrogen peroxide from a mouse osteoblastic cell line, MC3T3‐E1

FEBS Letters

Shibanuma, Motoko¹  Egawa, Kiyoshi¹  Yoshiwara, Mitsue¹  Nose, Kiyoshi¹ 

[1] Department of Microbiology, Showa University, School of Pharmaceutical Sciences, Hatanodai 1-5-8, Shinagawa-ku, Tokyo, Japan

关键词: Hydrogen peroxide;    ras-recision gene;   

DOI  :  10.1016/0014-5793(95)00957-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hydrogen peroxide appears to mediate growth factor actions, and it inhibits DNA synthesis in normal mouse osteoblastic cells (MC3T3-E1) at non-toxic doses. However the sensitivity of cells to H₂O₂ is greatly decreased in their ras-transformants. To understand the molecular basis of this sensitivity to H₂O₂, we attempted to identify H₂O₂-inducible cDNA clones from MC3T3 cells by differential screening of cDNA libraries, and one of such genes, named HIC-53, was isolated. The level of HIC-53 mRNA was moderately increased by H₂O₂ as well as by calcium ionophore or dexamethasone, but was not increased by the addition of serum, tumor promotimg phorbol ester, or epidermal growth factor. Among mouse organs, HIC-53 mRNA levels were higher in the kidney and lung, but were almost undetectable in the brain, heart, bone, muscle or spleen. In MC3T3 cells transformed with v-Ki-ras, the HIC-53 mRNA level was markedly decreased, and effect of H₂O₂ was abolished. Although the biological function of HIC-53 is unknown at present, the predicted amino acid sequence exhibited some similarity with bovine cardiac Na⁺/Ca⁺ exchanger. The nucleotide sequence of HIC-53 cDNA showed no significant similarity with other known gene sequences.

【 授权许可】

Unknown