Protein insertion into the endoplasmic reticulum of permeabilized cells

FEBS Letters

Graf, R.¹  Hofmann, M.W.¹  Quader, H.¹  Jadot, M.¹  Martoglio, B.¹ 

[1] Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, 69120 Heidelberg, Germany

关键词: Cell permeabilization;    Site-specific photocrosslinking;    Endoplasmic reticulum;    Protein translocation;   

DOI  :  10.1016/0014-5793(95)00881-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have established efficient translocation of newly synthesized proteins into the endoplasmic reticulum of permeabilized Mel Juso cells. By site-specific photo-crosslinking we show that translocating polypeptide chains contact the same components of permeabilized cells ER as in dog pancreas rough microsomes. This cellular assay system has the potential to overcome the limitations of isolated microsomes in investigating the molecular environment of a newly synthesized protein after they have left the ER translocation site.

【 授权许可】

Unknown