Oligomeric complexes involved in translocation of proteins across the membrane of the endoplasmic reticulum

FEBS Letters

Wang, Lin¹  Dobberstein, Bernhard¹ 

[1] Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, D-69120 Heidelberg, Germany

关键词: Endoplasmic reticulum;    Blue native PAGE;    Protein targeting;    Protein translocation;    ER;    endoplasmic reticulum;    RM;    rough microsomes;    PKRM;    puromycin/high salt treated RM;    RAMP;    ribosome-associated membrane proteins;    SRP;    signal recognition particle;    SR;    SRP receptor;    TRAMp;    translocating chain-associated membrane protein;    OST;    oligosaccharyl transferase;    TRAP;    translocon-associated protein;    PAGE;    polyacrylamide gel electrophoresis;    ECL;    enhanced chemiluminescence;   

DOI  :  10.1016/S0014-5793(99)01075-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Proteins involved in protein translocation across the membrane of the endoplasmic reticulum assemble into different oligomeric complexes depending on their state of function. To analyse such membrane protein complexes we fractionated proteins of mammalian rough microsomes and analysed them using blue native PAGE and immunoblotting. Among the proteins characterised are the Sec61p complex, the oligosaccharyl transferase (OST) complex, the translocon-associated protein (TRAP) complex, the TRAM and RAMP4 proteins, the signal recognition particle (SRP) and the SRP receptor (SR). Interestingly, the RAMP4 protein, SR and OST complex display more than one oligomeric form.

【 授权许可】

Unknown