Voltage‐dependent calcium channel β‐subunits in combination with α 1 subunits, have a GTPase activating effect to promote the hydrolysis of GTP by Gα o in rat frontal cortex

FEBS Letters

Brickley, Kieran¹  Page, Karen¹  Campbell, Veronica¹  Dolphin, Annette C.¹  Wade, Robert¹  Berrow, Nicholas¹ 

[1] Department of Pharmacology, Royal Free Hospital School of Medicine, Rowland Hill Street, London NW3 2PF, UK

关键词: Calcium channel β-subunit;    G-protein Go;    GTP hydrolysis;    DHP;    dihydropyridine;    PTX;    pertussis toxin;    VDCC;    voltage-dependent calcium channel;   

DOI  :  10.1016/0014-5793(95)00813-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The dihydropyridine-sensitive calcium channel agonist (−)-BayK 8644 was found to produce an enhancement of the intrinsic hydrolysis of GTP by G_o in rat frontal cortex membranes. An anti-calcium channel β-subunit antiserum abolished the (−)-BayK 8644-stimulated hydrolysis of GTP by G_o and reduced the dihydropyridine binding capacity of the cortical membranes. A peptide which mimics the β-subunit binding domain of the calcium channel complex, also attenuated (−)-BayK 8644 activation of GTPase. This study suggests that the calcium channel β-subunit is the principal component of the channel complex involved in linking dihydropyridine agonist binding to enhanced hydrolysis of GTP by G_o. This may be a mechanism by which calcium channels can normally act to limit the duration of a G-protein modulatory signal.

【 授权许可】

Unknown