Partial restoration of inactivated ribosomes with sodium borohydride or amino acids

FEBS Letters

Li, Qing¹  Liu, Wang-yi¹  Ling, Jin¹ 

[1] Shanghai Institute of Biochemistry, Academia Sinica, 320 Yue-yang Road, Shanghai 200031, People's Republic of China

关键词: Amino acid;    Protein synthesis;    Ribosomal RNA;    RNA N-glycosidase;    Sodium borohydride;   

DOI  :  10.1016/0014-5793(95)00794-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The aldehyde radical of ribose C′₁ at position 4324 in rat liver 28S rRNA generated by RNA N-glycosidase was either reduced to an hydroxyl group by sodium borohydride or converted into aldimine through a nucleophilic addition of amino acid used as a primary amine. Analysis of the R-fragment of 28S rRNA by polyacrylamide gel electrophoresis showed that the reduction of aldehyde to an hydroxyl group with sodium borohydride was highly specific. The protein synthesis activity of modified ribosomes was partially restored with the removal of the active aldehyde by sodium borohydride or amino acid. Reduction of aldehyde with sodium borohydride restored 43.1 % of the protein synthesis activity. Among the twenty natural amino acids tested, tryptophan and histidine could restore 57.4% and 42.1 % of the ribosome activity when brome mosaic virus RNA was used as mRNA. We came to the conclusion that the active aldehyde radical at position 4324 of 28S rRNA in modified ribosome may cause the inactivation of the ribosome for protein synthesis.

【 授权许可】

Unknown