cDNA analysis of the mite allergen Lep d 1 identifies two different isoallergens and variants

FEBS Letters

van Hage-Hamsten, Marianne¹  van der Ploeg, Ingeborg¹  Olsson, Susanna¹  Schmidt, Margit¹ 

[1] Department of Laboratory Medicine, Division of Clinical Immunology, Karolinska Hospital, S-171 76 Stockholm, Sweden

关键词: Allergy;    Mite;    cDNA cloning;    PCR;    Polymorphism;   

DOI  :  10.1016/0014-5793(95)98164-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

For the first time the complete cDNA encoding two isoallergens of the non-pyroglyphid dust mite Lepidoglyphus destructor, Lep d 1, allergen has been sequenced. In addition, one of the isoallergens was found to have two variants. Oligonucleotides were designed from known amino acid sequences. The cDNA sequences were obtained by hybridizing these primers to mRNA and enhancement by the RT-PCR technique. To obtain the different complete encoding cDNA sequences and eliminate heteroduplex artifacts, we performed PCR + 1 reactions. Comparison of the amino acid sequence of the allergen shows leader sequences of 16 amino acids for both isoforms.

【 授权许可】

Unknown