Selective inhibition of gastrulation in the starfish embryo by albuside B, an inosine analogue

FEBS Letters

Myotoishi, Yuki¹  Isomura, Haruhiko¹  Kaneko, Hiroyuki²  Dan-Sohkawa, Marina²  Ikegami, Susumu¹  Hamada, Keiichi¹  Shimizu, Takahiko¹ 

[1] Department of Applied Biochemistry, Faculty of Applied Biological Sciences, Hiroshima University, 1-4-4 Kagmniyama, Higashi-Hiroshima, Hiroshima 739, Japan;Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan

关键词: Gastrula;    Mesendoderm;    Albuside B;    Soybean agglutinin;    Asterina pectinifera;   

DOI  :  10.1016/0014-5793(95)00751-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

External application of 0.2–100 μg/ml albuside B inhibits gastrulation of the starfish (Asterina pectinifera) embryo. Treated embryos retain the late blastula morphology with the vegetal plate. However, the vegetal plate is unreactive to soybean agglutinin, a probe for observing the progenitor cells of the archenteron (mesendoderm) in a normal embryo. The effective period of the treatment is limited from 4 to 6 h after fertilization, a period immediately before the onset of blastulation. RNA synthesis is unaffected during the period of sensitivity. The selectivity of the inhibition shows that albuside B may be a useful tool for studying the mechanisms of mesendoderm differentiation.

【 授权许可】

Unknown