Evidence for, and characterization of, a lipopolysaccharide‐inducible adenosine A2 receptor in human tracheal gland serous cells

FEBS Letters

Figarella, C.¹  Kammouni, W.¹  Merten, M.D.¹ 

[1] Groupe de Recherche sur les Glandes Exocrines, Faculté de Médecine, 27 Bld Jean Moulin, 13385 Marseille 05, France

关键词: Airways;    Human trachea;    Submucosa;    Secretion;    Cell culture;   

DOI  :  10.1016/0014-5793(95)00748-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human tracheal glands are considered as the principle secretory structures in the bronchotracheal tree. In earlier studies, we successfully performed primary cultures of human tracheal gland (HTG) serous cells and noted that these cells were responsive to many secretagogues including purinergic agonists but not to the inflammatory mediator adenosine. In this study, we demonstrate that adenosine was capable of inducing stimulation of protein secretion by HTG serous cells which had previously been cultured in pro-inflammatory conditions (induced by lipopolysaccharide (LPS)). This stimulation was inhibited by 8-phenyltheophylline but not by dipyridamole, which is indicative of a P1 purinoceptor. This inducible receptor is of the adenosine A2 subtype [rank potency order: 5′-(N-ethyl)-carboxamidoadenosine (NECA) > adenosine > N ⁶-(phenylisopropyl)-adenosine (PIA); and stimulation of adenylyl cyclase]. The adenosine-induced protein secretion was concentration-dependent, however, increased intracellular cyclic adenosine monophosphate (cAMP) was not dependent on the concentration of adenosine. The adenosine-induced secretion and the ATP-induced secretion were shown to be additive. This study concludes that there is evidence of a LPS-inducible adenosine A2 receptor in human tracheal gland serous cells.

【 授权许可】

Unknown