Influence of synthetic peptides, corresponding to fragments of the human α‐2 interferon molecule, on the proliferation of lymphoblastoid cells in vitro. Growth inhibition and receptor binding

FEBS Letters

Makarov, E.²  Makarova, N.¹  Kushch, A.¹  Romashkova, J.³  Danilkovich, A.³  Targoni, O.³  Freze, K.³  Valujskikh, A.³ 

[1] D.I. Ivanovsky Institute of Virology, Russian Academy of Medical Sciences, Moscow, Russian Federation;Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russian Federation;Department of Cellular and Immunology, Biological Faculty, Moscow State University, Moscow, Russian Federation

关键词: Synthetic peptide;    Interferon α;    Tumor cell;    Growth inhibition;    Receptor;    IFN;    interferon;    [3H]TdR;    [3H]thymidine;    K d;    equilibrium dissociation constant;    IC50;    concentration of competing ligand causing half-maximum displacement;    K i;    inhibition constant;   

DOI  :  10.1016/0014-5793(95)00731-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Influence of nine synthetic peptides from the C-terminal part of human α-2 interferon (EN) molecule on growth of a human T-lymphoblastoid cell line MT-4 was investigated. It was shown that some peptides inhibited the proliferation of MT-4 cells. It was also found that MT-4 cells expressed specific receptors on the outer surface of the plasma membrane. All studied peptides competed for binding to a common binding site. Antiproliferative activity of the peptides correlated with their length and affinity to receptors on the outer membrane of MT-4 cells.

【 授权许可】

Unknown