COPII: a membrane coat that forms endoplasmic reticulum‐derived vesicles

FEBS Letters

Barlowe, Charles¹ 

[1] Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03750, USA

关键词: Intracellular transport;    Vesicle budding;    Coat protein;    Endoplasmic reticulum;   

DOI  :  10.1016/0014-5793(95)00618-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vesicle budding from the endoplasmic reticulum (ER) has been reconstituted with washed membranes and three soluble proteins: Sec13 complex, Sec23 complex and the small GTPase Sar1p. The proteins that drive this cell-free vesicle budding reaction form an ∼10 nm thick electron dense coat on ER-derived vesicles. Although the overall mechanism of membrane budding driven by various cytoplasmic coats appears similar, the constituents of this new membrane coat are molecularly distinct from the non-clathrin coat (COP) involved in intra-Golgi transport and the clathrin-containing coats. The new vesicle coat has been termed COPII.

【 授权许可】

Unknown