FEBS Letters | |
COPII: a membrane coat that forms endoplasmic reticulum‐derived vesicles | |
Barlowe, Charles1  | |
[1] Department of Biochemistry, Dartmouth Medical School, Hanover, NH 03750, USA | |
关键词: Intracellular transport; Vesicle budding; Coat protein; Endoplasmic reticulum; | |
DOI : 10.1016/0014-5793(95)00618-J | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Vesicle budding from the endoplasmic reticulum (ER) has been reconstituted with washed membranes and three soluble proteins: Sec13 complex, Sec23 complex and the small GTPase Sar1p. The proteins that drive this cell-free vesicle budding reaction form an ∼10 nm thick electron dense coat on ER-derived vesicles. Although the overall mechanism of membrane budding driven by various cytoplasmic coats appears similar, the constituents of this new membrane coat are molecularly distinct from the non-clathrin coat (COP) involved in intra-Golgi transport and the clathrin-containing coats. The new vesicle coat has been termed COPII.
【 授权许可】
Unknown
【 预 览 】
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