Structure/function studies on cytoskeletal proteins in Dictyostelium amoebae as a paradigm

FEBS Letters

André, Bernadette¹  Andréoli, Christophe¹  Eichinger, Ludwig¹  Schleicher, Michael¹  Karakesisoglou, Jacques¹  Hofmann, Andreas¹  Noegel, Angelika A.²  Haugwitz, Michael¹  Stöckelhuber, Mechthild¹ 

[1] Institut für Zellbiologie, Schillerstr. 42, 80336 München, Germany;Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany

关键词: Actin-binding protein;    Cytoskeleton;    Cell motility;    Dictyostelium;    Molecular genetics;   

DOI  :  10.1016/0014-5793(95)00579-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The actin cytoskeleton in motile non-muscle cells is being regulated by a large number of actin-binding proteins. A deeper insight into the complex nature of the dynamic rearrangements of the microfilament system during cell movement requires an experimental system that allows the combined application of biochemical, biophysical, cell biological and molecular methods. Dictyostelium amoebae are well suited especially for a genetic approach because they are amenable to gene disruption, antisense and gene tagging techniques. The actin-binding proteins profilin, hisactophilin and protovillin are being described in this context as typical examples that either bind to G-actin, or anchor the actin cytoskeleton to the plasma membrane, or are structurally similar to vertebrate proteins but distinct in their functions.

【 授权许可】

Unknown