Calcium‐sensitive non‐muscle α‐actinin contains EF‐hand structures and highly conserved regions

FEBS Letters

Schleicher, Michael²  Noegel, Angelika¹  Witke, Walter¹ 

[1] >Max Planck Institutes for Biochemistry, 8033 Martinsried, FRG;Max Planck Institutes for Psychiatry, 8033 Martinsried, FRG

关键词: Actin-binding protein;    Cytoskeleton;    EF-hand structure;    Evolution;    Sequence homology;    (Dictyostelium discoideum);   

DOI  :  10.1016/0014-5793(87)80962-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The F-actin crosslinking molecule α-actinin from the slime mould Dictyostelium discoideum carries two characteristics EF-hand structures at the C-terminus. The calcium-binding loops contain all necessary liganding oxygens and most likely form the structural basis for the calcium sensitivity of strictly calcium-regulated non-muscle α-actinins. Furthermore, the sequence exhibits at the N-terminal site of the molecule a high degree of homology to chicken fibroblast α-actinin. This stretch of amino acids appears to have remained essentially constant during evolution and might represent the actin-binding site. The findings have led us to propose a model for the inhibitory action of Ca²⁺ on non-muscle α-actinins.

【 授权许可】

Unknown