Sphingomyelin is synthesized at the plasma membrane of oligodendrocytes and by purified myelin membranes: a study with fluorescent‐ and radio‐labelled ceramide analogues

FEBS Letters

Vos, Jan P.²  Marchesini, Sergio¹  Giudici, M.Luisa¹  van Golde, Lambert M.G.²  Lopes-Cardozo, Matthijs²  Magni, Paola¹  van der Bijl, Petra² 

[1] Department of Biomedical Sciences and Biotechnology, University of Brescia, Brescia, Italy;Laboratory of Veterinary Biochemistry, Utrecht University, Utrecht, The Netherlands

关键词: Sphingomyelin biosynthesis;    Myelin;    N-Lissamine rhodamine;    NBD;    Oligodendrocyte (rat);    BFA;    brefeldin A;    BSA;    bovine serum albumin;    CDM;    chemically defined medium;    Cer;    ceramides;    CerGlcT;    UDP-glucose;    ceramide glucosyltransferase;    CNPase;    2′;    3′-cyclic nucleotide 3′-phosphodiesterase;    DIC;    days in culture;    HBSS;    Hanks balanced salt solution;    HPTLC;    high-performance thin-layer chromatography;    LRh;    N-lissamine rhodamine;    NBD;    N-(7-nitrobenz-2-oxa-1;    3-diazol-4-yl);    PC;    phosphatidylcholine;    SM;    sphingomyelin;    SUV;    small unilamellar vesicles;   

DOI  :  10.1016/0014-5793(95)00695-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In most cell types sphingomyelin is synthesized predominantly in the cis-medial compartments of the Golgi stacks whereas the contribution of the plasma membrane is much lower. The aim of this study was to assess the contribution of both compartments to the synthesis of sphingomyelin in myelinating cells. Therefore, oligodendrocytes from rat spinal cord were incubated in culture with fluorescently- or radiolabelled ceramides, and the effects of a block in the vesicular flow (monensin, brefeldin A, low temperature) on surface synthesis of sphingomyelin were evaluated. The results indicate that ≈50% of the sphingomyelin synthase is present at the plasma and myelin membranes of oligodendrocytes.

【 授权许可】

Unknown