Angiotensin II transduces its signal to focal adhesions via angiotensin II type 1 receptors in vascular smooth muscle cells

FEBS Letters

Nakayama, Ichiro¹  Okuda, Masanori¹  Hoshijima, Masahiko¹  Kawahara, Yasuhiro¹  Yokoyama, Mitsuhiro¹ 

[1] Department of Internal Medicine (1st Division), Kobe University School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650, Japan

关键词: Angiotensin II;    Paxillin;    Focal adhesion kinase;    Tyrosine phosphorylation;    Vascular smooth muscle cell;   

DOI  :  10.1016/0014-5793(95)00693-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In cultured vascular smooth muscle cells (VSMCs), angiotensin II (Ang II) stimulated tyrosine phosphorylation of several proteins including a cluster of 70–80-kDa proteins as assessed by anti-phosphotyrosine immunoblotting. These 70–80-kDa proteins were identified as a focal adhesion-associated protein, paxillin, by anti-paxillin immunoprecipitation. Ang II-stimulated tyrosine phosphorylation of paxillin was detectable within 1 min and maximal at around 10 min and was concentration dependent (half-maximal effect at around 1 nM). Ang II also stimulated tyrosine phosphorylation of focal adhesion kinase in a time- and concentration-dependent manner. The Ang II type 1 (AT1) receptor antagonist, CV-11974, but not the Ang II type 2 receptor antagonist, PD123319, inhibited these reactions. These results indicate that Ang II transduces its signal to focal adhesions via AT1 receptors in cultured VSMCs.

【 授权许可】

Unknown