Diffuse vesicular distribution of Rab3D in the polarized neuroendocrine cell line AtT‐20

FEBS Letters

Martelli, Alberto M.¹  Baldini, Giulia²  Scherer, Philipp E.³  Bareggi, Renato¹  Baldimi, Giovanna¹  Lodish, Harvey F.³ 

[1] Dipartimento di Morfologia Umana Normale, via Manzoni 16, Trieste, I-34138, Italy;Department of Anatomy and Cell Biology, Columbia College of Physicians and Surgeons, 630 W 168 Street, New York, NY 10032, USA;Whitehead Institute for Biomedical Research, 9 Cambridge Center, Cambridge, MA 02142, USA

关键词: Low molecular weight GTP-binding protein;    Regulated secretion;    AtT-20 endocrine cell line;   

DOI  :  10.1016/0014-5793(95)00671-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The neuroendocrine cell line AtT-20 has two types of storage vesicles: dense core granules and synaptic vesicles, both sequestered at the tip of the processes. Here we show that Rab3D protein, which is abundant in fat cells, is also expressed in AtT-20 cells. Differently from Rab3A, which is localized in secretory vesicles accumulated at the tips, Rab3D has a diffuse vesicular distribution in the cytoplasm of the cell body, the processes and the tips. In AtT-20 cells, Rab3D may define a regulated secretory pathway which functions independently from cell polarity.

【 授权许可】

Unknown