Evidence for SecA‐ and ΔpH‐independent insertion of D1 into thylakoids

FEBS Letters

Robinson, Colin¹  van Wijk, Klaas Jan²  Knott, Tracy G.¹ 

[1] Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK;Arrhenius Laboratories, Department of Biochemistry, Stockholm University, S-106 91 Stockholm, Sweden

关键词: Chloroplast;    Thylakoid;    Protein transport;    Photosynthesis;    D1 protein;   

DOI  :  10.1016/0014-5793(95)00668-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Many nuclear-encoded proteins are targeted into chloroplast thylakoids by an azide sensitive Sec-related mechanism or by a ΔpH-driven mechanism. In this report, the requirements for the integration of chloroplast-encoded thylakoid proteins have been analysed in pulse-labeled intact chloroplasts. We show that the integration of the photosystem II reaction centre protein, D1, continues in the absence of a ΔpH and in the presence of azide. A range of other proteins are similarly targeted to thylakoids in the presence of azide, suggesting that the SecA-related mechanism is not widely used for the targeting of chloroplast-encoded proteins.

【 授权许可】

Unknown