Stimulation of cell proliferation and autoregulation of elastin expression by elastin peptide VPGVG in cultured chick vascular smooth muscle cells

FEBS Letters

Seyama, Yoshiyuki¹  Tajima, Shingo²  Wachi, Hiroshi¹  Yamashita, Sabrou¹  Okamoto, Kouji³  Suganami, Hideki³  Yamada, Haruyoshi²  Uemura, Yuko³ 

[1] Department of Clinical Chemistry, Hoshi College of Pharmacy, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142, Japan;Department of Dermatology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160, Japan;Department of Biochemical Engineering and Science, Kyushu Institute of Technology, 680-4 Kawazu, Iizuka, Fukuoka 820, Japan

关键词: Elastin fragment;    Elastin synthesis;    Smooth muscle cell;    BAPN;    β-aminopropionitril;    EDTA;    ethylenediaminetetraacetic acid;    NEM;    N-ethylmaleimide;    NMR;    nuclear magnetic resonance;    PMSF;    phenylmethylsulfonyl fluoride;    SDS-PAGE;    sodium dodecylsulfate-polyacrylamide gel electrophoresis;    SSC;    0.15 M sodium chloride and 0.015 M sodium citrate;    TCA;    trichloroacetic acid;    TPA;    12-O-tetradecanoylphorbol-13-acetate;   

DOI  :  10.1016/0014-5793(95)00641-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Synthetic elastin peptides, VPGVG or its polymer (VPGVG) _n , enhanced the proliferation of smooth muscle cells 1.5-fold during 48 h treatment at the concetrations over 10⁻⁶ M or 1.0 μg/ml, respectively. Monomeric and polymeric VPGVG sequences reduced elastin synthesis and its mRNA level to one-third and one-half of control respectively under the conditions in which the proliferation of cells were enhanced, but did not change collagen synthesis as measured by bacterial collagenase digestion. The elastin-specific autoregulation by elastin fragments may reflect the feedback regulation of elastin expression which may play an essential role in elastin metabolism under the normal and diseased conditions.

【 授权许可】

Unknown