Amylin and epinephrine have no direct effect on glucose transport in isolated rat soleus muscle

FEBS Letters

Rink, Timothy J.¹  Wolfe-Lopez, Deborah¹  Young, Andrew A.¹  Pittner, Richard A.¹ 

[1] Physiology Department, Amylin Pharmaceuticals, Inc., 9373 Towne Centre Drive, San Diego, CA 92121-3027, USA

关键词: Amylin;    Epinephrine;    Soleus;    Skeletal muscle;    Glucose transport;    2-Deoxyglucose;    3-O-Methylglucose;   

DOI  :  10.1016/0014-5793(95)00449-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Amylin and epinephrine did not significantly affect insulin stimulated, or basal, 3-O-methylglucose transport in isolated rat soleus muscle, as measured by the release of 3-O-methylglucose from pre-loaded tissue. Both amylin and epinephrine inhibited insulin-stimulated 2-deoxyglucose uptake (by 25% and 38%, respectively) in soleus muscle from fed rats but not from fasted rats. The latter results are consistent with amylin and epinephrine stimulating glycogenolysis and inhibiting hexokinase activity by intracellular accumulation of glucose 6-phosphate. We conclude that amylin, like epinephrine, does not specifically inhibit glucose transporters in skeletal muscle.

【 授权许可】

Unknown