Association of high molecular weight DNA fragmentation with apoptotic or non‐apoptotic cell death induced by calcium ionophore

FEBS Letters

Bessho, Rikimaru¹  Furusho, Kenshi¹  Akiyama, Yuichi¹  Kubota, Masaru¹  Kataoka, Akihiro¹  Usami, Ikuya¹  Okuda, Akiro¹  Wakazono, Yoshihiro¹ 

[1] Department of Pediatrics, Faculty of Medicine, Kyoto University, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-01, Japan

关键词: Apoptosis;    Necrosis;    High molecular weight DNA fragmentation;    Internucleosomal DNA fragmentation;    Endonuclease;   

DOI  :  10.1016/0014-5793(95)00405-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Calcium ionophore (A23187)-induced high molecular weight (HMW) and internucleosomal DNA fragmentation were investigated in human leukemia cell lines. An apoptosis-sensitive cell line, HL-60, showed HMW, internucleosomal DNA fragmentation and morphological changes of apoptosis by A23187. MOLT-4, which is resistant to apoptosis, exhibited only HMW DNA fragmentation and died of necrosis under the same conditions. Autodigestion experiments suggested the endonucleolytic activity to cause HMW fragmentation in the cytoplasm of both cell lines. The activity was more dependent on Mg²⁺ than Ca²⁺ in HL-60, whereas it was Ca²⁺-dependent in MOLT-4. These results suggest that HMW DNA fragmentation is not specific to apoptosis.

【 授权许可】

Unknown