Oligomerization state in solution of the cell cycle regulators p13suc1 from the fission yeast and p9cksphy from the myxomycete Physarum, two members of the cks family

FEBS Letters

Raynaud-Messina, Brigitte¹  Samamaa, Jean-Pierre²  Birck, Catherine² 

[1] Groupe de Pharmacologie du Cycle Cellulaire des Cellules Eucaryotes au Laboratoire de Pharmacologie et de Toxicologic Fondamentales du CNRS, UPR8221, 205 Route de Narbonne 31077-Toulouse, France;Groupe de Cristallographie Biologique du LPTF CEMESICNRS, 29 rue Jeanne Marvig, 31055 Toulouse cedex, France

关键词: Cell cycle regulation;    cdc2 kinase subunit;    cks protein;    Oligomerization;    X-ray solution scattering;    Schizosaccharomyces pombe;    Physarum polycephalum;    abr;    cdc2 kinase subunit;    SAXS;    small-angle X-ray scattering;    cdk;    cyclin-dependant kinase;    Rs;    Stokes radius;    Rs;    radius of gyration;    GA;    glutaraldehyde;   

DOI  :  10.1016/0014-5793(95)00300-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cks proteins (for cdc2 kinase subunit) are essential cell cycle regulators. They interact strongly with the mitotic cdc2 kinase, but the mechanism and the biological function of this association still await understanding. The oligomerization state in solution of two members of this ubiquitous protein family, the sucl gene product from the fission yeast and the newly cloned cksphy gene product from the myxomycete Physarum, was investigated by small-angle X-ray scattering (SAXS) and biochemical methods. We found that the major molecular species are monodispersed monomeric proteins. Minor amounts of dimeric sucl proteins were also found, but no equilibrium between the two forms was observed and surprisingly, the hexameric assemblies observed in the crystal structure of the human ckshs2 homolog were not detected. These apparent discrepancies between proteins that display cross-complementation address the question of the control of the cks oligomerization process and its link to the biological function.

【 授权许可】

Unknown