Isolation of human NuMA protein

FEBS Letters

Kalies, Ingrid²  Bischoff, F.Ralf¹  Kempf, Tore¹  Ponstingl, Herwig¹ 

[1] German Cancer Research Center, Division for Molecular Biology of Mitosis, Im Neuenheimer Feld 280, D-69009 Heidelberg, Germany;Universität Erlangen-Nürnberg, Institut und Poliklinik für Klinische Immunologie und Rheumatologie, Krankenhausstraße 12, D-91054 Erlangen, Germany

关键词: Chromatin condensation;    Centrosome;    RCC1;    Ran;    RanBP2;    Cell cycle regulation;   

DOI  :  10.1016/0014-5793(94)01151-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

NuMA is a protein involved in maintenance of nuclear structure and in the assembly of the mitotic spindle. Expression of amino-terminal deletion mutants results in a phenotype identical to that caused by a temperature-sensitive defect of RCC1 (regulator of chromosome condensation). Here we describe the isolation of NuMA protein from HeLa cells under mild conditions as a prerequisite to study its interactions with elements of the RCC1-Ran regulatory pathway. In an overlay assay, NuMA did not bind Ran·[γ-³²P]GTP. Thus it is clearly different from Ran·GTP binding proteins of similar M_r.

【 授权许可】

Unknown