Up‐regulation of ICAM‐1 expression on human dermal fibroblasts by IFN β in the presence of TNF‐α

FEBS Letters

Nakagawa, Hidemi²  Eguchi, Hiroaki³  Ezoe, Kyori¹  Hamaoka, Shuji³  Horikoshi, Takashi³  Hanada, Niro³ 

[1] Department of Plastic Surgery, School of Medicine, Sapporo Medical University, S1, W16, Chuoku, Sapporo 060, Japan;Department of Dermatology, Faculty of Medicine, Tokyo University, Honngo 7-3-1, Bunkyoku, Tokyo 113, Japan;Department of Dermatology, School of Medicine, Sapporo Medical University, S1, W16, Chuoku, Sapporo 060, Japan

关键词: IFN-β;    TNF-α;    ICAM-1;    Fibroblast;    IFN-β;    interferon-beta;    TNF-α;    tumor necrosis factoralpha;    PBMCs;    peripheral blood mononuclear cells;    ICAM-1;    intercellular adhesion molecule-1;    LFA-1;    leukocyte function associated molecule-1;    IL-1;    interleukin-;    FITC;    fluorescein isothiocyanate;   

DOI  :  10.1016/0014-5793(95)00299-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Unstimulated human fibroblasts show low or undetectable ICAM-1 expression. Interferon-beta (IFN-β) at concentrations of 10,100, and 1000 IU/ml in the presence of tumor necrosis factor-alpha (TNF-α) significantly increased the ICAM-I expression of fibroblasts in a dose-dependent manner. Treatment with IFN-β alone, however, did not up-regulate the ICAM-1 expression. Furthermore the attachment of peripheral blood mononuclear cells (PBMCs) to cytokine-treated fibroblasts was increased. This augmented attachment was partly inhibited by anti-ICAM-1 antibody. These results suggest that IFN-β and TNF-α may cooperatively modulate the attachment of PBMCs in the dermis.

【 授权许可】

Unknown