Adhesion molecules: a new target for immunoliposome‐mediated drug delivery

FEBS Letters

Bloemen, P.G.M.¹  van Bloois, L.²  Crommelin, D.J.A.²  Henricks, P.A.J.¹  van den Tweel, M.C.¹  Bloem, A.C.³  Storm, G.²  Nijkamp, F.P.¹ 

[1] Department of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, 3508 TB Utrecht, The Netherlands;Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, PO Box 80.082, 3508 TB Utrecht, The Netherlands;Department of Immunology, Academic Hospital Utrecht, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands

关键词: Immunoliposome;    Monoclonal antibody;    ICAM-1;    Epithelium;    Endothelium;    BSA;    bovine serum albumine;    CHOL;    cholesterol;    DMF;    dimethylformamide;    EDTA;    ethylene diaminetetraacetic acid;    EPG;    egg-phosphatidylglycerol;    FCS;    foetal calf serum;    GamFITC;    fluorescein isothiocyanate-labeled goat anti-mouse mAb;    HBS;    HEPES-buffered saline;    HUVEC;    human umbilical vein endothelial cells;    ICAM-1;    intercellular adhesion molecule-1;    IFN-γ;    interferon-γ;    MPB-PE;    N-[4-(p-maleimidophenyl)butyryl] phosphatidylethanolamine;    MFI;    mean fluorescence intensity;    mAb;    monoclonal antibody;    PBS;    phosphate-buffered saline;    PHEPC;    partially hydrogenated egg l-α-phosphatidylcholine;    PL;    phospholipid;    SATA;    N-succinimidyl S-acetylthioacetate;    TNF-α;    tumor necrosis factor-α;    VCAM-1;    vascular cell adhesion molecule-1;   

DOI  :  10.1016/0014-5793(94)01350-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The anti-ICAM-1 monoclonal antibody F10.2 was conjugated to liposomes to target to cells expressing the cell adhesion molecule ICAM-1. We demonstrate that F10.2 immunoliposomes bind to human bronchial epithelial cells (BEAS-2B) and human umbilical vein endothelial cells (HUVEC) in a specific, dose- and time-dependent manner. It appears that the degree of ICAM-1 expression is the limiting factor in the degree of immunoliposome binding to the cells. These results are a first step in the strategy for specific drug delivery to target sites characterised by increased expression of adhesion molecules.

【 授权许可】

Unknown