Biosynthetically lipid‐modified human scFv fragments from phage display libraries as targeting molecules for immunoliposomes

FEBS Letters

van Bloois, Louis¹  Logtenberg, Ton²  de Kruif, John²  Storm, Gert¹ 

[1] Department of Pharmaceutics, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.O. Box 80082, 3508 TB Utrecht, The Netherlands;Department of Immunology, HP F.03.821, Utrecht University, P.O. Box 85500 3508 GA Utrecht, The Netherlands

关键词: Phage display;    Lipid-modified scFv;    Immunoliposome;   

DOI  :  10.1016/S0014-5793(96)01335-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A human anti-CD22 single chain (sc) Fv antibody fragment from a synthetic phage antibody display library was biosynthetically lipid-tagged by using Escherichia coli lipoprotein sequences. The purified anti-CD22 scFv lipoprotein was incorporated into liposomes by detergent dilution. Anti-CD22 immunoliposomes were shown to bind specifically in a dose- and time-dependent manner to CD22+ cell lines and CD22+ B-lymphocytes present in freshly isolated samples of blood mononuclear cells. The immunoliposomes were demonstrated to accumulate in intracellular compartments. Biosynthetically lipid-tagged human scFv antibody fragments isolated from phage display libraries may facilitate the construction of immunoliposomes with improved properties.

【 授权许可】

Unknown