The Sec61 complex is essential for the insertion of proteins into the membrane of the endoplasmic reticulum

FEBS Letters

Rapoport, Tom¹  Görlich, Dirk³  High, Stephen²  Jungnickel, Berit¹  Oliver, Jason² 

[1] Max-Delbrück Center for Molecular Medicine, Robert Rössle Strasse 10, 01115 Berlin-Buch, Germany;School of Biological Sciences, University of Manchester, 2.205 Stopford Building, Oxford road, Manchester M13 9PT, UK;Wellcome/CRC Institute, Tennis Court Road, Cambridge, CB2 1QR, UK

关键词: Membrane protein biosynthesis;    Endoplasmic reticulum;    Reconstitution;   

DOI  :  10.1016/0014-5793(95)00223-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cross-linking studies have implicated Sec61α as the principal component adjacent to newly synthesised membrane proteins during insertion into the endoplasmic reticulum. Using proteoliposomes which have been reconstituted from purified components of the endoplasmic reticulum [Görlich, D and Rapoport, T.A., Cell 75 (1993) 615–630] we have found that the Sec61 complex, consisting of three subunits, is essential for the insertion of single-spanning membrane proteins. This is true for signal-anchor proteins of both orientations, and for proteins with a cleavable signal sequence. These results support the view that Sec61α is a major component of the ER translocation site and promotes both the insertion of membrane proteins and the translocation of secretory proteins.

【 授权许可】

Unknown