Adenovirus DNA replication in vitro is stimulated by RNA from uninfected HeLa cells

FEBS Letters

Kwant, M.M.¹  van der Vliet, P.C.¹  van Dam, D.¹ 

[1] Laboratory for Physiological Chemistry, State University of Utrecht, Vondellaan 24 A, 3521 GG Utrecht, The Netherlands

关键词: Adenovirus;    DNA replication in vitro;    Reconstitution;    Small RNA;   

DOI  :  10.1016/0014-5793(84)80449-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Adenovirus DNA replication was studied in a partially reconstituted system consisting of purified viral proteins (DNA-binding protein, precursor terminal protein and Ad DNA polymerase) and a nuclear extract from uninfected HeLa cells. Optimal DNA replication required the presence of a heat-stable, ribonuclease-sensitive fraction from the cytosol of uninfected cells. This fraction stimulated the initiation about 3-fold and the replication of origin fragments 5–10-fold. Sedimentation analysis indicated the presence of a fast-sedimenting and a slow-sedimenting component which complemented each other. At least part of the stimulation was caused by low-molecular-mass RNA.

【 授权许可】

Unknown