In vitro binding of Bacillus thuringiensis var. israelensis individual toxins to midgut cells of Anopheles gambiae larvae (Diptera: Culicidae)

FEBS Letters

Ravoahangimalala, Olga¹  Charles, Jean-François¹ 

[1] Bactéries Entomopathogènes, Institut Pasteur, 25 rue du Dr. Roux, 75724 Paris Cedex 15, France

关键词: Bacillus thuringiensis var. israelensis;    ϱ-Endotoxin;    Binding;    Anopheles gambiae;    Receptor;   

DOI  :  10.1016/0014-5793(95)00220-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Midguts from Anopheles gambiae fourth instars were dissected and processed for immuno-light microscopy. Cloned insecticidal crystal proteins (ICPs) from Bacillus thuringiensis var. israelensis (Bti) were individually expressed in crystal-negative strains of Bacillus thuringiensis. Tissue sections of A. gambiae were incubated in vitro with each solubilized and trypsin-activated ICP. Immunodetection of CryIVA, CryIVB, CryIVD and CytA toxins on sections was performed using purified rabbit IgG directed against BtiICPs, in combination with an anti-rabbit IgG/peroxidase. CryIVA, CryIVB, CryIVD and CytA toxins were detected on the apical brush border of midgut cells, in the gastric caecae and posterior stomach. CytA was also detected, to a lesser extent, on microvilli of anterior stomach cells.

【 授权许可】

Unknown