FEBS Letters | |
DNA binds neutrophil elastase and mucus proteinase inhibitor and impairs their functional activity | |
Belorgey, Didier1  Bieth, Joseph G.1  | |
[1] Laboratoire d'Enzymologie, INSERM U392, Université Louis Pasteur de Strasbourg, F-67400 Illkirch, France | |
关键词: Elastase; Mucus proteinase inhibitor; Enzyme kinetics; DNA; Cystic fibrosis; NE; neutrophil elastase; MPI; mucus proteinase inhibitor = secretory leucoprotease inhibitor (SLPI); Suc-Ala3-pNA; succinyl-Ala3-p-nitroanilide; MeOSuc-Ala2-Pro-Val-pNA; methoxysuccinyl-Ala2-Pro-Val-p-nitroanilide; RBB-elastin; remazol Brilliant-blue elastin; | |
DOI : 10.1016/0014-5793(95)00173-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
DNA binds neutrophil elastase and mucus proteinase inhibitor as evidenced by affinity chromatography on elastase-Sepharose, inhibitor-Sepharose and DNA-cellulose. DNA is a potent hyperbolic inhibitor of elastase. The polynucleotide-enzyme complex is partially active on synthetic substrates and on elastin. DNA strongly increases k diss and K i for the inhibition of elastase by mucus proteinase inhibitor. The above effects are all salt-dependent. At physiological ionic strength, DNA is a potent inhibitor of the elastolytic activity of elastase and increases k diss and K i for the elastase-mucus proteinase inhibitor interaction 160-fold and 100-fold, respectively.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO201912020300844ZK.pdf | 369KB | download |