期刊论文详细信息
FEBS Letters | |
Stoichiometry of K+/H+ antiport helps to explain extracellular pH 11 in a model epithelium | |
Wieczorek, Helmut1  Harvey, William R.1  Azuma, Masaaki1  | |
[1] Zoologisches Institüt der Universität, Postfach 202136, D-80021 München, Germany | |
关键词: K+/H+ antiport; V-ATPase; K+ transport; Alkalinization; Manduca sexta; MOPS; 3-morpholinopropanesulfonic acid; TMA; tetramethylammonium; | |
DOI : 10.1016/0014-5793(95)00146-Z | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The stoichiometry of K+/H+ antiport was measured fluorometrically by the static head method in highly purified vesicles from goblet cell apical membranes of larval lepidopteran midgut. The measured stoichiometry of 1 K+/2 H+ explains how the antiport results in electrophoretic exchange of extracellular H+ for intracellular K+, driven by the voltage component of the proton-motive force of an H+ translocating V-ATPase that is located in the same membrane. In turn, the exchange of K+ for H+ helps to explain how the midgut contents are alkalinized to a pH of 11.
【 授权许可】
Unknown
【 预 览 】
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RO201912020300821ZK.pdf | 366KB | download |