期刊论文详细信息
FEBS Letters | |
Chimeric alpha‐beta oligonucleotides as antisense inhibitors of reverse transcription | |
Debart, Françoise2  Rayner, Bernard2  Toulme, Jean-Jacques1  Boiziau, Claudine1  Imbach, Jean-Louis2  | |
[1] Laboratoire de Biophysique Moléculaire, INSERM U 386, Université Bordeaux II, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France;Laboratoire de Chimie Bioorganique, CNRS URA 488, Université de Montpellier II, Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France | |
关键词: RNase H; Antisense oligonucleotide; Moloney Murine Leukemia Virus; | |
DOI : 10.1016/0014-5793(95)00138-Y | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Alpha-beta chimeric 17-mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized. The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha-beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes. All chimeras inhibited in vitro cDNA synthesis in a cell-free system to various extent, via the degradation of the RNA target by RNase H.
【 授权许可】
Unknown
【 预 览 】
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