Chimeric alpha‐beta oligonucleotides as antisense inhibitors of reverse transcription

FEBS Letters

Debart, Françoise²  Rayner, Bernard²  Toulme, Jean-Jacques¹  Boiziau, Claudine¹  Imbach, Jean-Louis² 

[1] Laboratoire de Biophysique Moléculaire, INSERM U 386, Université Bordeaux II, 146 rue Léo Saignat, F-33076 Bordeaux Cedex, France;Laboratoire de Chimie Bioorganique, CNRS URA 488, Université de Montpellier II, Place Eugène Bataillon, F-34095 Montpellier Cedex 5, France

关键词: RNase H;    Antisense oligonucleotide;    Moloney Murine Leukemia Virus;   

DOI  :  10.1016/0014-5793(95)00138-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Alpha-beta chimeric 17-mer oligodeoxyribonucleotides containing either 5, 10 or 15 beta nucleotides were synthesized. The stability of the RNA/chimera hybrids was only slightly affected by the alpha stretch and by the alpha-beta link, as was the affinity of the Moloney Murine Leukemia Virus reverse transcriptase for the duplexes. All chimeras inhibited in vitro cDNA synthesis in a cell-free system to various extent, via the degradation of the RNA target by RNase H.

【 授权许可】

Unknown