Effects of mutations at position 36 of tRNAGlu on missense and nonsense suppression in Escherichia coli

FEBS Letters

Gregory, Steven T.¹  Dahlberg, Albert E.¹ 

[1] Section of Biochemistry, Box G, Brown University, Providence, RI 02912, USA

关键词: Suppressor tRNA;    Translational efficiency;    tRNAGlu;    Anticodon;   

DOI  :  10.1016/0014-5793(95)00132-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mutations in the anticodon of tRNA^Glu (UUC) were isolated or constructed and characterized for their ability to suppress cognate nonsense or missense mutations in vivo. The C36-to-A36 transversion mutation was isolated as an ochre and an amber suppressor, while the G36 transversion was selected as a CAG missense suppressor. tRNA^Glu suppressors of an AAG missense mutation could not be isolated, and a U36 transition mutation introduced into tRNA^Glu in vitro conferred no suppressor phenotype. Over-expression of glutamyl-tRNA synthetase did not increase the activity of the U36 mutant tRNA^Glu, suggesting a defect at the level of translation rather than at the level of synthetase recognition.

【 授权许可】

Unknown