Sequence and functional characterization of RNase P RNA from the chl a/b containing cyanobacterium Prochlorothrix hollandica

FEBS Letters

Fingerhut, Christiane¹  Schön, Astrid¹ 

[1] Institut für Biochemie, Bayerische Julius-Maximilians-Universität, Biozentrum, Am Hubland, 97074 Würzburg, Germany

关键词: RNase P;    Ribozyme;    Pre-tRNA processing;    tRNAGlu;    Cyanobacterium;    Prochlorothrix hollandica;   

DOI  :  10.1016/S0014-5793(98)00519-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Only a few complete sequences and very limited functional data are available for the catalytic RNA component of cyanobacterial RNase P. The RNase P RNA from the chl a/b containing cyanobacterium Prochlorothrix hollandica belongs to a rarely found structural subtype with an extended P15/16 domain. We have established conditions for optimal in vitro ribozyme activity, and determined the kinetic parameters for cleavage of pre-tRNA^Tyr. Analysis of pre-tRNA mutants revealed that the T-stem sequence only plays a modulating role, whereas the CCA end is essential for efficient product formation.

【 授权许可】

Unknown