Stimulation of vgf gene expression by NGF is mediated through multiple signal transduction pathways involving protein phosphorylation

FEBS Letters

Volonté, Cinzia²  D'Arcangelo, Gabriella¹  Salton, Stephen R.J³ 

[1] Department of Neurobiology and Behavior, State University of New York at Stony Brook, Stony Brook, NY 11794-5230, USA;Department of Pathology and Center for Neurobiology and Behavior, College of Physicians and Surgeons, Columbia University, New York, NY 10032, USA;Fishberg Research Center for Neurobiology, Mt. Sinai School of Medicine, Box 1065, One Gustave Levy Place, New York, NY 10029-6574, USA

关键词: VGF;    NGF;    Trk;    PC12 cell;    Protein kinase N;    Protein phosphorylation;    2-AP;    2-aminopurine;    bFGF;    basic fibroblast growth factor;    6-MMPR;    6-methylmercaptopurine riboside;    NGF;    nerve growth factor;    ODC;    ornithine decarboxylase;    PLC-γ1;    phospholipase C-γ1;    PKA;    protein kinase A;    PKC;    protein kinase C;    PKN;    protein kinase N;    6-TG;    6-thioguanine;   

DOI  :  10.1016/0014-5793(95)00086-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The vgf gene encodes one of the most rapidly induced neuronal mRNAs identified in NGF-treated PC12 cells. Maximal inhibition of VGF mRNA induction was achieved using K-252a, an inhibitor of the NGF-receptor Trk tyrosine kinase, and by mutating both Y490 (SHC association site) and Y785 (PLC-γ1 association site) of Trk. Inhibitors of the NGF-activated protein kinase N (PKN) were found to partially and in some cases transiently block VGF induction by NGF while in PKA-deficient PC12 cells, VGF induction by NGF was comparable to that observed in parental PC12 cells. The binding of NGF to Trk therefore activates redundant signal transduction pathways which converge to regulate vgf gene expression.

【 授权许可】

Unknown