Influence of endogenous GM1 ganglioside on TrkB activity, in cultured neurons

FEBS Letters

Kuriyama, Masaru²  Mutoh, Tatsuro²  Pitto, Marina¹  Palestini, Paola¹  Masserini, Massimo¹  Ferraretto, Anita¹ 

[1] Department of Medical Chemistry and Biochemistry, University of Milano, Via Saldini 50, I-20133 Milano, Italy;2nd Department of Internal Medicine, Fukui University Faculty of Medicine, 910-1193 Fukui, Japan

关键词: Brain-derived neurotrophic factor tyrosine kinase receptor;    GM1 ganglioside;    Brain-derived neurotrophic factor;    Cerebellar granule cell;    Detergent-resistant membrane domain;    BDNF;    brain-derived neurotrophic factor;    CTB;    cholera toxin B subunit;    ECL;    enhanced chemiluminescence;    HRP;    horseradish peroxidase;    NGF;    nerve growth factor;    NT;    neurotrophins;    PKC;    protein kinase C;    PMA;    phorbol-12-myristate-13 acetate;    Trk;    tyrosine kinase;    TrkB;    brain-derived neurotrophic factor tyrosine kinase receptor;    TyrP;    phosphotyrosine;   

DOI  :  10.1016/S0014-5793(98)01344-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We verified the hypothesis that changes in the endogenous GM1 ganglioside density in the environment of TrkB, receptor of brain-derived neurotrophic factor, can affect receptor activity, and focused on rat cerebellar granule cells expressing both GM1 and TrkB. Changes of the amount of GM1 associated to immunoprecipitated TrkB and of receptor tyrosine phosphorylation were evaluated after treatment with phorbol-12-myristate-13-acetate (1 μM, 7 min), reported to affect the plasma membrane distribution of endogenous gangliosides in the same cells. After treatment, the amount of GM1 associated to receptor and TrkB phosphorylation decreased by about 40%. The amount of associated GM1 decreased by about 33% also after concomitant treatment with phorbol ester and brain-derived neurotrophic factor, but in this case the neurotrophin was unable to enhance receptor tyrosine phosphorylation. These results for the first time suggest that changes in the amount of endogenous GM1 in the environment of TrkB can modulate receptor activity, and offer new clues for a better understanding of physiological and pathological events of the nervous system.

【 授权许可】

Unknown