The use of electrospray mass spectrometry to identify an essential arginine residue in type II dehydroquinases

FEBS Letters

Pitt, Andrew R.¹  Coggins, John R.²  Krell, Tino² 

[1] Department of Pure and Applied Chemistry University of Strathclyde, Glasgow G1 1XL, Scotland, UK;Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow G12 8QQ, Scotland, UK

关键词: Type II dehydroquinase;    Phenylglyoxal;    Electrospray mass spectrometry;    DHQase;    dehydroquinase;    ESMS;    electrospray mass spectrometry;    GdnHCl;    guanidine hydrochloide;    HPLC;    high performance liquid chromatography;    MaxEnt;    maximum entropy;    PGO;    phenylglyoxal;   

DOI  :  10.1016/0014-5793(95)00083-L

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The arginine-specific reagent phenylglyoxal has been used to identify a hyper-reactive arginine residue which is essential for activity in the type II dehydroquinases of Streptomyces coelicolor and Aspergillus nidulans. Electrospray mass spectrometry was used both to characterise the phenylglyoxal modified protein, and to identify the phenylglyoxal modified peptides following enzymatic digestion. The advantages of using electrospray mass spectrometry for monitoring arginine modication aimed at identifying functional residues in proteins are discussed.

【 授权许可】

Unknown