Substrate specificity of rat liver mitochondrial carnitine palmitoyl transferase I: evidence against α‐oxidation of phytanic acid in rat liver mitochondria

FEBS Letters

Poulos, Alfred¹  Singh, Harmeet¹ 

[1] Department of Chemical Pathology, Women's and Children's Hospital, 72 King William Road, North Adelaide, SA 5006, Australia

关键词: Fatty acid;    Phytanic acid;    Pristanic acid;    Peroxisome;    Mitochondrion;    Transport;    CPT;    carnitine palmitoyl transferase or carnitine acyltransferase;    COT;    carnitine octanoyl transferase;    TLC;    thin layer chromatography;   

DOI  :  10.1016/0014-5793(95)00035-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The two branched chain fatty acids pristanic acid (2,6,10,14-tetramethylpentadecanoic acid) and phytanic acid (3,7,11,15-tetramethylhexadecanoic acid) were converted to co-enzyme A thioesters by rat liver mitochondrial outer membranes. However, these branched chain fatty acids could not be converted to pristanoyl and phytanoyl carnitines, respectively, by mitochondrial outer membranes. As expected, the unbranched long chain fatty acids, stearic acid and palmitic acid, were rapidly converted to stearoyl and palmitoyl carnitines, respectively, by mitochondrial outer membranes. These observations indicate that the branched chain fatty acids could not be transported into mitochondria. The data presented strongly suggest that in rat liver, α-oxidation of phytanic acid occurs in organelles other than mitochondria.

【 授权许可】

Unknown