FEBS Letters | |
‘Brain‐type’ N‐glycosylation of asialo‐transferrin from human cerebrospinal fluid | |
Hoffmann, Andrea1  Nimtz, Manfred2  Getzlaff, Rita2  Conradt, Harald S.1  | |
[1] Department of Molecular Biology, GBF- Gesellschaft fur Biotechnologische Forschung, Mascheroder Weg 1, 38124 Braunschweig, Germany;Department of Molecular and Instrumental Structural Research, GBF - Gesellschaft fur Biotechnologische Forschung, Mascheroder Weg 1, 38124 Braunschweig, Germany | |
关键词: N-Glycosylation (‘brain-type’); Cerebrospinal fluid; Human transferrin; Human β-trace protein; CSF; cerebrospinal fluid; deoxyHex; deoxyhexose; Fuc; fucose; Gal; galactose; GlcNAc; N-acetylglucosamine; Hex; hexose; HexNAc; N-acetylhexosamine; HexNAc-ol; N-acetylhexosaminitol; HPAEC; high-pH anion-exchange chromatography; HPLC; high-performance liquid chromatography; MALDI/TOF-MS; matrix-assisted laser desorption/ionization time of flight mass spectrometry; NeuNAc; N-acetylneuraminic acid; PNGase F; polypeptide-N 4-(N-acetyl-glucosaminyl)asparagine amidase F; | |
DOI : 10.1016/0014-5793(95)00034-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Asiolo-transferrin from human cerebrospinal fluid was purified to homogeneity. Investigation of the structural characteristics of its oligosaccharides support our hypothesis of ‘brain-type’ glycosylation of intrathecally synthesized cerebrospinal fluid proteins. For carbohydrate structural analysis, high-pH anion-exchange chromatography, methylation analysis, liquid secondary ion- and matrix-assisted laser desorption/ ionization mass spectrometry of the permethylated derivatives were used. The major structure turned out to be a complex-type agalacto-diantennary oligosaccharide with bisecting N-acetylglucosamine and proximal fucose. Analysis of a second transferrin preparation containing both asialo- and sialo-transferrin revealed another major glycan species derived from the sialylated transferrin variant which is galactosylated and lacks bisecting N-acetylglucosamine and fucose.
【 授权许可】
Unknown
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