‘Brain‐type’ N‐glycosylation of asialo‐transferrin from human cerebrospinal fluid

FEBS Letters

Hoffmann, Andrea¹  Nimtz, Manfred²  Getzlaff, Rita²  Conradt, Harald S.¹ 

[1] Department of Molecular Biology, GBF- Gesellschaft fur Biotechnologische Forschung, Mascheroder Weg 1, 38124 Braunschweig, Germany;Department of Molecular and Instrumental Structural Research, GBF - Gesellschaft fur Biotechnologische Forschung, Mascheroder Weg 1, 38124 Braunschweig, Germany

关键词: N-Glycosylation (‘brain-type’);    Cerebrospinal fluid;    Human transferrin;    Human β-trace protein;    CSF;    cerebrospinal fluid;    deoxyHex;    deoxyhexose;    Fuc;    fucose;    Gal;    galactose;    GlcNAc;    N-acetylglucosamine;    Hex;    hexose;    HexNAc;    N-acetylhexosamine;    HexNAc-ol;    N-acetylhexosaminitol;    HPAEC;    high-pH anion-exchange chromatography;    HPLC;    high-performance liquid chromatography;    MALDI/TOF-MS;    matrix-assisted laser desorption/ionization time of flight mass spectrometry;    NeuNAc;    N-acetylneuraminic acid;    PNGase F;    polypeptide-N 4-(N-acetyl-glucosaminyl)asparagine amidase F;   

DOI  :  10.1016/0014-5793(95)00034-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Asiolo-transferrin from human cerebrospinal fluid was purified to homogeneity. Investigation of the structural characteristics of its oligosaccharides support our hypothesis of ‘brain-type’ glycosylation of intrathecally synthesized cerebrospinal fluid proteins. For carbohydrate structural analysis, high-pH anion-exchange chromatography, methylation analysis, liquid secondary ion- and matrix-assisted laser desorption/ ionization mass spectrometry of the permethylated derivatives were used. The major structure turned out to be a complex-type agalacto-diantennary oligosaccharide with bisecting N-acetylglucosamine and proximal fucose. Analysis of a second transferrin preparation containing both asialo- and sialo-transferrin revealed another major glycan species derived from the sialylated transferrin variant which is galactosylated and lacks bisecting N-acetylglucosamine and fucose.

【 授权许可】

Unknown