Prohibitin, an antiproliferative protein, is localized to mitochondria

FEBS Letters

Fiedler, Klaus¹  Simons, Kai¹  Ikonen, Elina¹  Parton, Robert G.¹ 

[1] Cell Biology Programme, European Molecular Biology Laboratory, Postfach 102209, 69012 Heidelberg, Germany

关键词: Prohibitin;    Mitochondria;    Antiproliferative;    Carsinogenesis;    cDNA;    complementrary DNA;    PCR;    polymerase chain reaction;    BHK;    baby hamster kidney;    FCS;    fetal calf serum;    PBS;    phosphate-buffered saline;    SDS-PAGE;    sodium dodecyl sulphate-polyacrylamide gel electrophoresis;   

DOI  :  10.1016/0014-5793(94)01444-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Prohibitin is a ubiquitously expressed protein with antiproliferative properties. When rat prohibitin tagged with a carboxy-terminal c-Myc epitope was expressed in baby hamster kidney cells the protein was targeted to mitochondria. In immunofluorescence microscopy prohibitin colocalized with a mitochondrial marker E3. Immunoelectron microscopy revealed that prohibitin was associated with the periphery of mitochondria. The amino-terminus of prohibitin shares characteristics of the known mitochondrial import signals, and positioning of the tag at the N-terminus causes accumulation of the protein in the cytoplasm. These findings help to direct functional studies on prohibitin and suggest that a mitochondrial protein may act as a tumor suppressor.

【 授权许可】

Unknown