FEBS Letters | |
Prohibitin, an antiproliferative protein, is localized to mitochondria | |
Fiedler, Klaus1  Simons, Kai1  Ikonen, Elina1  Parton, Robert G.1  | |
[1] Cell Biology Programme, European Molecular Biology Laboratory, Postfach 102209, 69012 Heidelberg, Germany | |
关键词: Prohibitin; Mitochondria; Antiproliferative; Carsinogenesis; cDNA; complementrary DNA; PCR; polymerase chain reaction; BHK; baby hamster kidney; FCS; fetal calf serum; PBS; phosphate-buffered saline; SDS-PAGE; sodium dodecyl sulphate-polyacrylamide gel electrophoresis; | |
DOI : 10.1016/0014-5793(94)01444-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Prohibitin is a ubiquitously expressed protein with antiproliferative properties. When rat prohibitin tagged with a carboxy-terminal c-Myc epitope was expressed in baby hamster kidney cells the protein was targeted to mitochondria. In immunofluorescence microscopy prohibitin colocalized with a mitochondrial marker E3. Immunoelectron microscopy revealed that prohibitin was associated with the periphery of mitochondria. The amino-terminus of prohibitin shares characteristics of the known mitochondrial import signals, and positioning of the tag at the N-terminus causes accumulation of the protein in the cytoplasm. These findings help to direct functional studies on prohibitin and suggest that a mitochondrial protein may act as a tumor suppressor.
【 授权许可】
Unknown
【 预 览 】
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