Wortmannin inhibits carcinogen‐stimulated phosphorylation of ethanolamine and choline

FEBS Letters

Tomono, Masahiro¹  Kiss, Zoltan¹ 

[1] The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: 7;    12-Dimethylbenz[a]anthracene;    Ethanolamine;    Choline;    Kinase;    Wortmannin;    Phosphatidylinositol-3-kinase;    Etn;    ethanolamine;    EtnP;    ethanolamine phosphate;    Cho;    choline;    ChoP;    choline phosphate;    DMBA;    7;    12-dimethylbenz[a]anthracene;    PtdEtn;    phosphatidylethanolamine;    PtdCho;    phosphatidylcholine;    PI3K;    phosphatidylinositol-3-kinase;   

DOI  :  10.1016/0014-5793(94)01434-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have previously reported that in C3H/10T1/2 fibroblasts the environmental carcinogen 7,12-dimethyl-benz[a]anthracene (DMBA) stimulated phosphorylation of ethanolamine (Etn). Here we show that in these fibroblasts DMBA also stimulates phosphorylation of choline (Cho). Wortmannin (50–200 nM), an established inhibitor of phosphatidylinositol-3-kinase (PI3K), significantly inhibited DMBA-induced phosphorylation of both Etn and Cho. Wortmannin also inhibited the effect of insulin, a major activator of PI3K, on DNA synthesis. However, insulin had no effect on the phosphorylation of Etn and Cho. These data suggest that a carcinogen-induced kinase phosphorylates both Etn and Cho, and that the inhibitory effect of wortmannin on Etn/Cho kinase activity may be unrelated to its inhibitory effect on PI3K activity.

【 授权许可】

Unknown