Ha‐Ras stimulates uptake and phosphorylation of ethanolamine: inhibition by wortmannin

FEBS Letters

Crilly, Karan S.¹  Kiss, Zoltan¹ 

[1] The Hormel Institute, University of Minnesota, 801 16th Avenue NE, Austin, MN 55912, USA

关键词: Ras;    Ethanolamine;    Choline;    Kinase;    NIH 3T3 fibroblasts;    Phosphatidylinositol 3-kinase;    Ha-Ras;    Harvey-Ras;    Etn;    ethanolamine;    EtnP;    ethanolamine phosphate;    Cho;    choline;    ChoP;    choline phosphate;    PI3K;    phosphatidylinositol 3-kinase;    PtdEtn;    phosphatidylethanolamine;    PtdCho;    phosphatidylcholine;    PMA;    phorbol 12-myristate 13-acetate;   

DOI  :  10.1016/0014-5793(94)01371-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transformation of NIH 3T3 fibroblasts by Ha-Ras resulted in large increases in the phosphorylation of both [¹⁴C]ethanolamine (Etn) and [¹⁴C]choline (Cho) when these precursors were added to the medium. Wortmannin, an inhibitor of phosphatidylinositol 3-kinase (P13K), preferentially decreased phosphorylation of externally added Etn in the Ha-Ras transformed, but not in the untransformed, fibroblasts. However, wortmannin had no effect on the phosphorylation of Etn formed endogenously by phorbol ester-stimulated hydrolysis of phosphatidylethanolamine. Data suggest that interaction of mutated Ras with PI3K leads to specific stimulation of Etn uptake, followed by nearly quantitative phosphorylation of Etn by a Ras-activated Cho/Etn kinase.

【 授权许可】

Unknown