Changes in ascorbate peroxidase activities during fruit ripening in Capsicum annuum

FEBS Letters

Guillemaut, Pierre¹  Schantz, Marie-Luce²  Schreiber, Hélène¹  Schantz, Rodolphe² 

[1] Laboratoire de Biologie Végétale Appliquée, IUT Louis-Pasteur, 3 Rue de l'Argonne, 67000 Strasbourg, France;Institut de Biologie Moléculaire des Plantes, C.N.R.S., Université Louis Pasteur, 12 Rue du Général Zimmer, 67084 Strasbourg Cedex, France

关键词: Ascorbate peroxidase;    Fruit-ripening;    Isozyme;    Organelle;    cDNA Sequence;    Capsicum annuum;   

DOI  :  10.1016/0014-5793(94)01413-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ascorbate peroxidase (APX) system was studied in Capsicum annuum during fruit ripening. A large increase in organelle APX activity was found during chloroplast-chromoplast transition whereas only a slight difference was detected in total fruit extracts. On native gels, four different isoforms were found in total fruit extracts but the patterns for red and green fruit were quite different. In isolated organelles, six isozymes were found and a comparison of the patterns showed significant differences. A cDNA encoding a cytosolic APX was cloned and sequenced. The corresponding transcript was shown to increase 3–4-fold during fruit ripening.

【 授权许可】

Unknown