FEBS Letters | |
Chaos in DNA partition during the last mitoses of the proliferative life‐span of human fibroblasts | |
Macieira-Coelho, A.1  | |
[1] INSERM and University of Paris VI, 73 bis rue Maréchal Foch, 78000 Versailles, France | |
关键词: Human fibroblast; Aging; Senescence; Terminal differentiation; Quantal mitosis; | |
DOI : 10.1016/0014-5793(94)01407-R | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
We have followed with autoradiography the course of DNA synthesis and with cytofluorometry the partition of DNA during mitosis, through the different phases of the life-span of human fibroblasts. Towards the end of the proliferative potential of the cell population, DNA synthesis was disturbed during the late part of the S period. The DNA content of metaphases and of anaphases had a normal Gaussian distribution during the whole proliferative life-span of the cell population. At the very end, however, when cells entered the post-mitotic phase, the distribution of DNA content deviated from the normal Gaussian probability, becoming significantly skewed.
【 授权许可】
Unknown
【 预 览 】
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