期刊论文详细信息
FEBS Letters
Stimulation of transforming growth factor‐β 1 transcription by cyclosporine
Sharma, V.K.1  Prashar, Y.1  Sehajpal, P.1  Suthanthiran, M.1  Khanna, A.1 
[1] The Rogosin Institute, Department of Transplantation and Extracorporeal Therapy, Departments of Medicine, Biochemistry and Surgery, The New York Hospital-Cornell Medical Center, 430 E 71st Street, New York, NY 10021, USA
关键词: Cyclosporine;    TGF-β;    Transcription;    CAT activity;    Mobility shift assay;    Transcription factor;   
DOI  :  10.1016/0014-5793(94)01382-B
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

In searching for a candidate mechanism for the immunosuppressive as well as fibrogenic consequences of cyclosporine usage, we have explored the hypothesis that cyclosporine stimulates transcription of transforming growth factor-β 1 (TGF-β 1), a multifunctional cytokine endowed with immunosuppressive and fibrogenic properties. Our results demonstrate that cyclosporine (i) stimulates TGF-β 1 promoter-dependent transcription of chloramphenicol acetyl transferase gene in transiently transfected human A-549 cells, (ii) stimulates the synthesis of TGF-β 1 RNA transcripts in human T cells, and (iii) permits the expression/emergence of DNA regulatory proteins (retinoblastoma control factor-1 (RCF-1) and RCF-2) that bind and regulate TGF-β 1 promoter activity. Our studies demonstrate for the first time that cyclosporine stimulates TGF-β 1 gene transcription and suggest a novel mechanism of action of cyclosporine.

【 授权许可】

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