Hydrogen peroxide preferentially enhances the tyrosine phosphorylation of epidermal growth factor receptor

FEBS Letters

Shimizu, Nobuyoshi¹  Gamou, Shinobu¹ 

[1] Department of Molecular Biology, Keio University School of Medicine, Tokyo 160, Japan

关键词: EGF receptor;    Protein phosphorylation;    Hydrogen peroxide;    Oxidant stress;    EGF;    epidermal growth factor;    DMEM;    Dulbecco's modified Eagle medium;    FBS;    fetal bovine serum;   

DOI  :  10.1016/0014-5793(94)01335-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We found that hydrogen peroxide (H₂O₂) enhances EGF receptor tyrosine phosphorylation in intact cells as well as solubilized membrane of an EGF receptor hyperproducing cell line NA. An antioxidant MnCl₂ effectively inhibited this enhancement. Interestingly, overall phosphorylation of the EGF receptor enhanced by H₂O₂ was half that of the EGF-enhanced phosphorylation when the receptor immunoprecipitated from [³²P]ortho-phosphate-labeled cells was examined. Tryptic phospho-peptide mapping of these receptors revealed that EGF enhanced the phosphorylation on five specific residues including serine 671, 1,046 and 1,047, threonine 669 and tyrosine 1,173, whereas H₂O₂ enhanced the phosphorylation remarkably on tyrosine 1,173 and three other residues and only moderately on serine 1,046 and 1,047 and threonine 669. Thus, H₂O₂ preferentially enhances the tyrosine phosphorylation of EGF receptor through oxidant stress.

【 授权许可】

Unknown